FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_sv118.35100000057741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_sv118.35100000057741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38724424
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC8580772.215854779402271TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT951100.043.05681631
CGATGTA956200.043.00151434
ACCGATG951950.042.98291832
CGTCTGA971850.042.96858616
CTCGTAT958050.042.92232542
ACACGTC976600.042.9117613
TATCTCG961450.042.8652739
TCTCGTA960800.042.8636641
CCGATGT955850.042.8357933
GATCGGA994450.042.8080981
ATCGGAA993900.042.7956472
ACGTCTG979600.042.74118815
TCGTATG963100.042.72295843
GTCACCG961300.042.6280429
CGTATGC965400.042.56562444
TCACCGA963000.042.48970830
ATCTCGT971750.042.40860740
TCGGAAG1001400.042.4073373
CACGTCT995850.042.129614
CGGAAGA1007400.042.0822534