FastQCFastQC Report
Mon 6 Jun 2016
C8CR7ACXX_l01n01_bgm25.351000000578b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8CR7ACXX_l01n01_bgm25.351000000578b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36952317
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGC16200.014.99961326
CGGTCCG5950.014.36955336
CGAACTC40500.013.55520616
TCGAACT41050.013.2091615
CTCGAAC41550.012.56285414
AGACGCG6500.011.7739131
AGACCCG24650.011.6882181
ATCCGCC20800.011.68240727
TCTCGTA18500.011.55395941
CTCGTAT17900.011.06188642
AAATCCG24150.010.71400925
TCGTATG18600.010.4036243
GCCTCGA49050.010.3667612
GACGCGT3704.1763997E-910.3384392
AGACGCT25250.010.2516031
CCTCGAA52500.010.02832613
AGACCAG252850.09.8991331
GGCCTCG50550.09.88110411
CGCGATA2751.1528959E-59.81794330
TCACGTC220150.09.65795131
Produced by FastQC (version 0.11.2)