FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_sea_174.3510000004e517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_sea_174.3510000004e517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21496900
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC895090.4163809665579688TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC117450.036.3939129
GTATGCC122200.035.1658145
ACGTCTG132000.034.8535915
CACACGT135700.034.1029312
CGTCTGA139000.033.1145616
CGTATGC132350.032.58791744
CACGTCT142800.032.3278914
GCACACG144800.032.08401511
AGTCACA135750.031.75300428
CAGTCAC141200.031.10099627
TCACACT139150.030.81540130
TCGTATG141800.030.60655643
GTCTGAA151200.030.51701417
CTCGTAT146050.029.91618242
AGCACAC158950.029.66737410
ACACTTG146700.028.89262832
ACACGTC163550.028.2401213
CACACTT154250.027.78465531
CTGAACT167350.027.73330719
TCTGAAC168000.027.67957118
Produced by FastQC (version 0.11.2)