FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r9.3510000004e871.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r9.3510000004e871.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8063009
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG155523819.288555922485017TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATTTCGTATG245240.3041544415986637TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG241450.29945396315445016TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTGAAACGATCTCGTATG136800.16966370743229978TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTGAAACGATCTCGTATG112520.1395508798266255TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTGAAACGATCTCGTATG108310.13432950403503208TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAA1789000.044.2956317
TCTGAAC1777050.044.29092418
CACACGT1803200.044.28546512
TCGTATG1761450.044.25605845
CGTCTGA1784600.044.23717516
CACGTCT1789100.044.22525414
ACGTCTG1788700.044.18105715
ACACGTC1795000.044.14255513
AACTCCA1772650.044.13688322
ACTCCAG1773250.044.12195223
CTGAACT1783750.044.077919
AGCACAC1816750.044.05505410
CTCCAGT1774500.044.0439624
GAACTCC1781750.044.03141821
TGAACTC1796300.044.0141720
CAGTCAC1788750.043.98864727
CCAGTCA1783750.043.97447626
AGTCACG1797300.043.9621428
GAGCACA1832050.043.9154139
GCACACG1821050.043.89708711
Produced by FastQC (version 0.11.2)