FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r8.3510000004e7f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r8.3510000004e7f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6254218
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC100404116.053821596880695TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1105950.043.61800412
GTCTGAA1098000.043.60973417
CACGTCT1104900.043.5776614
ACGTCTG1104600.043.56505215
CGTCTGA1102400.043.53771216
TCTGAAC1097750.043.53563718
GTATGCC1078700.043.48345645
ACACGTC1109050.043.42879513
AGAGCAC1117550.043.413638
AGCACAC1113650.043.41310510
GAGCACA1116350.043.3696069
AACTCCA1097750.043.29995722
CTGAACT1102600.043.26864619
AAGAGCA1122350.043.2600367
ACTCCAG1099850.043.23978423
CTCCAGT1100400.043.19772324
TGAACTC1104450.043.1839520
GAACTCC1102850.043.1813221
CCAGTCA1101200.043.11118326
GCACACG1120500.043.09176611
Produced by FastQC (version 0.11.2)