FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r7.3510000004e772.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r7.3510000004e772.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6206505
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG3502485.643240438862129TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGAAC443000.043.60872318
CACACGT444850.043.54909512
AGTCACC435900.043.5293728
GTCTGAA444950.043.5035617
CGTCTGA445000.043.45317516
CAGTCAC438350.043.44004427
AACTCCA442150.043.3770922
CACGTCT445850.043.37033514
ACTCCAG441850.043.34034723
CTCCAGT441450.043.32865524
ACGTCTG446600.043.3226815
TCGTATG398200.043.26978745
CCAGTCA440250.043.26790226
ACACGTC448500.043.1742713
GTCCCGA411750.043.12996336
CTGAACT448900.043.0656319
AGCACAC451000.043.0357510
GAACTCC447350.043.03380221
TGAACTC448900.042.9954720
CACCCGT437700.042.9340231
Produced by FastQC (version 0.11.2)