FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r6.3510000004e6fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r6.3510000004e6fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7938551
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG5748547.241296302058147TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTGAA680950.043.64670617
AGTCACA671600.043.61788628
TCTGAAC678000.043.57447418
CACACGT682300.043.55484812
CACGTCT680750.043.5339414
TCGTATG652400.043.5286645
CGTCTGA682650.043.4622116
AACTCCA673350.043.44438622
TGTCAGA667750.043.42519435
CTCCAGT673550.043.3947424
ACGTCTG684700.043.37808615
CAGTCAC676550.043.36193527
ACTCCAG673750.043.34847323
GTCACAT675900.043.32042729
CCAGTCA674350.043.3132426
AGCACAC687350.043.30821610
TCACATG675300.043.3022830
CTGAACT682000.043.2760219
TCAGAAT669150.043.22947737
AGAGCAC692850.043.2128528
Produced by FastQC (version 0.11.2)