FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r5.3510000004e680.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r5.3510000004e680.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5692270
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG1839693.2319092383179293TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACAGT201750.043.3436930
AGTCACA203850.043.2834428
GTCACAG204600.043.03680829
ACAGTCA203200.042.9240632
CACAGTC203950.042.75480331
AGTCAAC202850.042.69867334
TCGTATG204650.042.64566445
CAGTCAC208100.042.54000527
CAGTCAA205100.042.38382333
GTCTGAA212150.042.37476717
ACTCCAG209900.042.35740723
ACAATCT204200.042.35400439
AACTCCA210450.042.35360722
TCTGAAC212000.042.33046318
CTCCAGT209750.042.2911724
CTCGTAT206000.042.28973444
CACGTCT213050.042.24855414
CGTCTGA212650.042.24339316
CCAGTCA210150.042.1464426
CACACGT214100.042.11602412