FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r4.3510000004e616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r4.3510000004e616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525790
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCTC2601.8007995E-1013.84521538
CACGTCT5600.012.85576314
TCTGAAC4750.012.788118
GTCTGAA4950.012.72590717
CTCGTAT4000.012.37416242
TCGTATG4300.012.03406843
CACACGT5300.011.88645812
GTAATCT2851.0455551E-811.84130337
AGCACAC5850.011.53813710
CTCCAGT5900.011.43944924
ACGTCTG6200.011.24879315
GTAACTG1600.001109688411.24879323
AACTCCA6050.011.155827522
CGTCTGA6100.011.06438516
CAGTCAC4152.1827873E-1110.8422127
GTATGCC4601.8189894E-1210.76014145
GGGACTC1500.00878895910.49887329
ACTCCAG6050.010.41210623
AGAGCAC6950.010.3594218
TGTAATC3603.007699E-89.99932236
Produced by FastQC (version 0.11.2)