FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r3.3510000004e59e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r3.3510000004e59e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4836647
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC283890.5869562116069252TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36100.037.64338742
CAGTCAC36750.037.21977627
TCTGAAC38150.036.85640718
GTCTGAA38700.036.5651417
TCGTATG37400.036.3349343
CCAGTCA38150.036.20773326
GTATGCC37850.035.9623845
TCTCGTA37950.035.92690741
ACTCCAG39000.035.7070223
AACTCCA39350.035.44659422
AGTCACG38350.035.31495728
CTCCAGT39550.035.2104624
CACACGT41050.034.47402212
CGTCTGA41200.034.45558516
AGATCTC39200.034.43726338
CACGTCT41200.034.4009814
CTGAACT41800.033.7995519
CAGATCT40150.033.56674637
ACGTCTG43050.033.02717615
CGTATGC41250.032.9982144
Produced by FastQC (version 0.11.2)