FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r13.3510000004ea49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r13.3510000004ea49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8509243
Sequences flagged as poor quality0
Sequence length51
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC1390841.6345049730040615TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCAT159800.042.37652635
TAGCATC160500.041.9989236
GGTAGCA162900.041.57009534
ACGGTAG165750.041.22179432
AGTCACG168800.041.0362628
CACACGT171400.041.031412
CTCGTAT166750.040.72159242
GTCTGAA171100.040.66871317
TCGTATG168050.040.6071743
GTATGCC168050.040.5803945
CACGTCT173550.040.57422614
CAGTCAC170650.040.5650227
ACTCCAG172350.040.30848723
TCTGAAC172500.040.28648418
AACTCCA171900.040.16534822
TCTCGTA169500.040.10074241
CCAGTCA172700.040.08350426
CGTCTGA173850.040.06422816
ACGTCTG175900.040.00657715
CACGGTA171850.039.83689531