FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r12.3510000004e9dd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r12.3510000004e9dd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9548452
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC3118663.26614198825108TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT364800.041.96735412
CACGTCT365650.041.7638914
AGTCACC356250.041.76118528
GTACGAT353600.041.6294535
CAGTCAC361950.041.56348427
GTCTGAA366450.041.5315117
TCTGAAC366750.041.436218
AGAGCAC371950.041.3999828
AACTCCA365100.041.3769822
AGCACAC370650.041.373710
CGTCTGA368750.041.3639816
ACGTCTG369350.041.3150615
CACCGTA358900.041.29677631
CCAGTCA365200.041.27984226
ACTCCAG366200.041.24040623
ACCGTAC359050.041.19180332
CTCGTAT357750.041.18621442
GAGCACA374950.041.026739
AAGAGCA375700.040.9148867
CTCCAGT369150.040.88646324
Produced by FastQC (version 0.11.2)