FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r11.3510000004e957.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r11.3510000004e957.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7017257
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG1015001.4464341266110106TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT116900.040.99587644
TCGTATG116450.040.9031245
GTCTGAA124050.040.805117
AGTCACG122750.040.79738628
CACACGT124500.040.7307612
TCTGAAC123900.040.70923618
CAGTCAC123400.040.69187527
GGAATCT118050.040.5586839
CACGTTT121500.040.53316531
AACTCCA123800.040.46953622
CACGTCT126150.040.26849414
ACTCCAG124450.040.2400923
CGTCTGA126150.040.17932516
CCAGTCA125500.040.1185326
CTCCAGT125500.040.04682524
ACGTTTC122550.040.02094732
TCTCGTA120250.039.9286343
ACGTCTG127800.039.66057215
CTGAACT127200.039.65310319
TTTCGGA122900.039.2845735
Produced by FastQC (version 0.11.2)