FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_r10.3510000004e8eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_r10.3510000004e8eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4895804
Sequences flagged as poor quality0
Sequence length51
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG535531.0938550644592797TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATCT59100.043.1729639
TTATCTC60100.042.4171740
GGCCTTA60650.042.18004236
GCCTTAT61750.041.46509637
CCTTATC61700.041.46223438
AGTCACG64000.040.9515328
AACTCCA66200.040.44018622
CTCGTAT64100.040.12125444
ACTCCAG67000.039.92373723
CAGTCAC66050.039.88487227
GTCTGAA68050.039.6713817
TCTGAAC68150.039.54714218
CCAGTCA66950.039.48311626
CTCCAGT67850.039.25780524
TCGTATG65650.039.2082645
CGTCTGA69400.039.0293416
TCTCGTA66500.038.70710443
CACACGT69900.038.62220812
CACGTCT70000.038.56624614
CTGAACT70200.038.52046619
Produced by FastQC (version 0.11.2)