FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_51.3510000004e49f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_51.3510000004e49f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6372022
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG11900.026.2790611
ACACGTC12000.025.68429613
GAACTCC11800.025.35700621
ACTCCAG11950.024.09741423
CACACGT12900.023.71871812
GAGCACA13500.023.3329879
CGTCTGA14450.021.640916
ACGTCTG15500.020.17490415
CACGTCT15550.019.82067914
AGCACAC16100.019.70405810
CAGTCAC12900.019.35805327
GATCGGA16650.019.0675371
GTATGCC13050.017.58511745
CTGAACT17700.017.54018619
CGCGGTT6550.017.1877271
GTCTGAA18350.017.16407217
CTCCAGT17150.017.05326724
TCTGAAC18850.016.94748718
GCGATAA14650.016.2913861
CATCTCG13300.016.23959539
Produced by FastQC (version 0.11.2)