FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_50.3510000004e418.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_50.3510000004e418.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12447819
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1096890.8811905121692403TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG123750.041.2900139
ACTCCAG127000.041.2743123
GAACTCC128650.040.7974121
GCACACG130650.040.75916311
CACACGT131050.040.68625612
GAGCACA133050.040.1445549
ACACGTC132550.040.0892413
CCGATGT126850.040.06488433
CACGTCT134350.039.46840314
GTATGCC131950.039.38826845
CGTCTGA134600.039.32824316
CAGTCAC132350.039.2659127
CGTATGC133000.039.04347644
ACGTCTG135950.039.003915
AGCACAC138400.038.55866210
AACTCCA139000.037.9052822
CTCGTAT137100.037.85946342
CTGAACT140400.037.655519
GTCTGAA141400.037.5642117
TCTCGTA138000.037.53103341
Produced by FastQC (version 0.11.2)