FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_49.3510000004e3ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_49.3510000004e3ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10500652
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC799420.7613051075304658TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG98200.042.0626311
ACTCCAG98400.041.42762423
GAACTCC99300.041.2560521
CACACGT100500.041.10000212
GAGCACA101650.040.8369379
ACACGTC101400.040.71224613
AGTCACA97500.040.2640828
GTCACAT97850.039.9821129
AGCACAC104400.039.75935710
GTATGCC100700.039.7244845
GATCGGA105350.039.433691
CAGTCAC101550.039.36719527
CGTCTGA105800.038.87026616
ACGTCTG106300.038.75092315
CGTATGC103200.038.7185644
CACGATC99450.038.55003736
GATCTCG101450.038.4995539
CACGTCT107600.038.38728314
TCACGAT100900.038.26144435
ATCACGA101300.038.1325734
Produced by FastQC (version 0.11.2)