FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_44.3510000004e326.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_44.3510000004e326.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12977102
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC5790244.4618898734093335TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCTC644250.043.5329638
AATATCT646550.043.25265537
CTCGTAT650200.043.22439242
TATCTCG652300.043.1271339
TCGTATG657050.043.01004443
TCTCGTA654100.042.97682641
TCTGAAC663000.042.9380918
CCAATAT649700.042.91446735
AGTCACG661300.042.85116228
GTCTGAA669400.042.739317
GTATGCC661750.042.66716845
CAGTCAC666000.042.5251127
CGTCTGA672200.042.50772516
CCAGTCA665350.042.431426
CTCCAGT666200.042.3266124
CGTATGC667350.042.3192544
ACGTCTG676650.042.26474415
ATCTCGT665600.042.2378340
CAATATC660750.042.10518636
CACGTCT680200.042.04085514