FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_43.3510000004e2ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_43.3510000004e2ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15027660
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC9491856.316252829781882TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACA1036650.043.32153728
GTATGCC1040500.043.2485945
ACACAGT1032700.043.23278832
TCTGAAC1054550.043.21553818
TCACACA1038150.043.17659430
CTCGTAT1041400.043.07942642
GTCTGAA1061050.043.0377317
ACAGTGA1035850.042.966834
GTCACAC1044100.042.95855729
TCGTATG1048100.042.94571743
TCTCGTA1044950.042.9266141
CGTCTGA1066400.042.87666716
CACACAG1047900.042.7622731
CACAGTG1045150.042.7307133
CAGTCAC1055200.042.73052627
ACGTCTG1072950.042.69040315
CTCCAGT1058500.042.65044824
CCAGTCA1056300.042.61787426
CACGTCT1077750.042.56289714
CAGTGAT1048750.042.4983635