FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_42.3510000004e227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_42.3510000004e227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20936104
Sequences flagged as poor quality0
Sequence length51
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC242389511.577583871383137TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2657250.043.7985545
AGTCACT2696850.043.7091628
CTCGTAT2668200.043.5968842
TCTGAAC2726350.043.55556518
TCTCGTA2675050.043.5180441
CCAATCT2644750.043.516537
TCGTATG2679000.043.5151943
CAGTCAC2708550.043.46387527
GTCTGAA2739300.043.4605317
GACCAAT2655300.043.43950335
ACCAATC2647900.043.43074836
AATCTCG2679650.043.41079739
CCAGTCA2707300.043.40666226
CGTCTGA2737900.043.3972916
CTCCAGT2711200.043.38488424
CAATCTC2668350.043.36224438
TCACTGA2719300.043.33114230
GAACTCC2729700.043.314221
TGAACTC2741150.043.27853820
GTCACTG2725700.043.27789329