FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_matt_41.3510000004e1af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_matt_41.3510000004e1af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13825838
Sequences flagged as poor quality0
Sequence length51
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3047392.204126795062983TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG323600.044.32093431
ACTTAGG322700.044.3120832
TCACTTA326050.044.0291430
TTAGGCA322650.044.0121534
CTTAGGC324350.043.85761333
CTCGTAT330250.043.31501442
TAGGCAT330150.043.046435
TCGTATG334300.043.00562743
AGTCACT336100.042.9065528
TCTCGTA334050.042.86269841
GTCACTT338700.042.5107629
CAGTCAC347250.041.6584227
CGTATGC347400.041.40984744
TCTGAAC351850.041.3821918
GTATGCC348700.041.28127745
CCAGTCA351250.041.2672926
ATCTCGT349750.040.9387740
GTCTGAA356850.040.88432317
GCATCTC348000.040.67914638
CTCCAGT357200.040.63641724