FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_chip873.3510000004eb3b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_chip873.3510000004eb3b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10113822
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC755240.7467404508404438TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC84300.041.0185431
ACTAGCT86050.040.15820332
TCACTAG87800.039.48531330
TAGCTTA88300.038.95695534
CTAGCTT88850.038.8930533
CTCGTAT90700.038.4502542
TCGTATG93850.037.30335643
CGTATGC95800.036.63799344
TCTCGTA96150.036.294241
ACACGTC100600.036.02674513
GTATGCC97200.036.01769345
CACGTCT101000.035.77269414
TATCTCG97500.035.65373639
CACACGT101600.035.60625512
CAGTCAC98800.035.54458627
AGCTTAT97700.035.30090735
CTTATCT98100.035.114437
GAACTCC103000.034.70676821
CGTCTGA104250.034.5711616
ACGTCTG104450.034.5695815
Produced by FastQC (version 0.11.2)