FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l08n01_chip864.3510000004eab2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l08n01_chip864.3510000004eab2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20598948
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG227620.11050078868105302TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG54050.022.55928615
CGTATCT48500.022.2205939
CCGTATC49650.021.84175138
CACACGT56800.021.54669812
ACACGTC57950.021.19634413
GCACACG58450.021.05392311
CACGTCT59800.020.46536314
CTCGTAT55150.020.27525544
CGTCTGA61850.019.8234216
TCGTATG57350.019.49747845
GTTCCGT56700.019.2835335
GTCACAG57800.019.0718229
CCAGTCA62500.018.57353626
CAGTCAC60700.018.53131327
AGTTCCG60250.018.40870334
TCCGTAT59900.018.36723737
ACTCCAG64800.018.3655323
GTATCTC59450.018.31681840
GAACTCC68350.017.77366421
TCCAGTC68400.017.13580325
Produced by FastQC (version 0.11.2)