FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea202.3510000004e7b5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea202.3510000004e7b5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5801496
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT9200.010.5147714
ACACGTC8650.010.40310613
GCACACG8450.010.383100511
ACGTCTG9150.09.8346315
CGTATGC7400.09.12131344
CACACGT9750.08.99868612
CGTCTGA10800.08.74872316
GTCACCG7350.08.57017829
GTATGCC8200.08.50580945
AGTCACC8400.07.766723628
TCGTATG9200.07.58126543
CGATGTA9950.07.46225134
AGCACAC13400.07.38717210
ACTCCAG13150.07.014185423
GAACTCC12550.06.99101121
TCCAGTC12250.06.794926625
CCAGTCA11300.06.768923826
CTCGTAT10003.6379788E-126.749771642
CAGTCAC11500.06.455579827
CCGATGT10508.0035534E-116.214021733