FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea201.3510000004e73f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea201.3510000004e73f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5534775
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC12150.016.66426813
GCACACG12500.015.8377211
CACGTCT14000.015.10496914
CACACGT13650.014.99784112
ACGTCTG13300.014.71592715
CGTCTGA13900.014.56624916
CGTATGC10600.014.43397144
CTCGTAT11300.014.33629242
GTATGCC11650.013.32618845
GAACTCC15750.012.85529121
CCAGTCA15150.012.32495926
CACGATC11700.012.30847836
ACGATCT13250.012.05737437
ACTCCAG15400.011.97879623
TCCAGTC16200.011.66498725
TCGTATG13750.011.61818943
GATCGGA18050.011.4735421
GATCTCG14800.011.40296139
CGATCTC13650.011.37435238
CAGTCAC14950.011.28627