FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea199.3510000004e83e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea199.3510000004e83e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432124
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC161010.662013943368019TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG19300.041.26509532
CACTTAG19650.040.64458531
CACACGT20500.039.72671512
CTCGTAT20050.039.4995342
ACACGTC21050.038.58185213
TTAGGCA20700.038.367934
TCGTATG20950.038.01744543
TAGGCAT20900.038.00074435
TCTCGTA20950.037.9100541
CACGTCT21700.037.42617414
GCACACG21950.037.3073911
CAGTCAC21450.037.23308627
CGTATGC21550.037.16776344
GTATGCC21750.036.82598545
TCGGAAG22200.036.714023
CGTCTGA23050.035.6245916
ATCTCGT22400.035.55795340
ACGTCTG23050.035.5269915
GAACTCC22800.035.52185421
CATCTCG22250.035.2927839
Produced by FastQC (version 0.11.2)