FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea197.3510000004e630.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea197.3510000004e630.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13551305
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG29450.018.79164911
ACGTCTG31850.018.0113315
ACACGTC31700.017.74172413
CACGTCT32050.017.40759314
CTCGTAT30350.017.19825744
TCGTATG29600.017.1779745
CACACGT32850.017.05214512
CGTCTGA34300.016.52804416
CAGTCAC35750.014.15874427
CCAGTCA36950.014.00334126
GAACTCC40950.013.29463821
ACTCCAG40050.013.20019723
AGCACAC43300.012.98898510
TCTCGTA42000.012.80281143
GATCGGA43650.012.7908181
ACATGTC40800.012.57255132
TCCAGTC41650.012.53115925
ATGTCAG42100.012.29174934
CATGTCA44050.011.8493833
CACATGT46150.011.60226431