FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea195.3510000004e531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea195.3510000004e531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11959117
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC7299546.1037449504006025TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC797000.043.735213
CACACGT798800.043.70705412
CACGTCT797850.043.64348614
GCACACG803100.043.54306811
GTATGCC787850.043.536345
ACGTCTG802050.043.38128715
CGTCTGA802550.043.303816
CTCGTAT788150.043.288542
CCTTGTA793850.043.22316733
AGCACAC809900.043.21968510
AGTCACC795550.043.20974728
CGTATGC794150.043.18242644
TCGTATG795000.043.1560743
GATCGGA816600.043.123361
GTCACCT797800.043.09634429
TCGGAAG816200.043.07253
CAGTCAC800350.043.03774327
ACCTTGT798800.042.9912232
GAACTCC805450.042.94678521
GTAATCT793350.042.91139237