FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea194.3510000004e4b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea194.3510000004e4b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11629303
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC302670.2602649531102595TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCTA43200.032.49807732
CGTATGC45150.031.4442744
GGCTACA45450.030.74194534
CACGGCT45600.030.73712331
CGGCTAC45800.030.70272833
CTCGTAT46500.030.57975842
AGTCACG47550.030.18502228
ACACGTC50400.030.12968313
GTCACGG47700.029.66563429
GTATGCC48200.029.50121945
TCGTATG48150.029.48512543
CACACGT51450.029.42734112
GCACACG52500.029.09590111
CACGTCT52750.029.00065414
ACGTCTG53250.028.55935915
GCTACAT48750.028.47646335
TCACGGC50200.027.9641930
CAGTCAC50950.027.90578727
CGTCTGA56600.027.02799416
GAACTCC56450.026.82084721
Produced by FastQC (version 0.11.2)