FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea192.3510000004e44f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea192.3510000004e44f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10680337
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1566161.4663956764660142TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG185700.041.02044728
CGTATGC185600.040.87763644
GTATGCC185400.040.8731945
CTCGTAT185600.040.62306242
TCGTATG187150.040.56312643
GTCACGA191100.039.69650329
CAGTCAC192300.039.62426827
ACACGTC198000.039.08558313
CACGTCT197900.039.07122814
CCAGTCA195750.039.02934626
TCTCGTA193850.038.9758141
GAACTCC198250.038.8320321
CAGATCT193800.038.7090437
ACGTCTG199550.038.7030715
CACACGT200450.038.65275212
CACGATC195300.038.62503431
AGATCTC195550.038.61577238
TCCAGTC199300.038.3905825
ACTCCAG200350.038.38027223
GCACACG203050.038.31292711
Produced by FastQC (version 0.11.2)