FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea191.3510000004e3c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea191.3510000004e3c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8860791
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC90337810.195229748675937TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1001650.044.03142513
CACACGT1002450.044.0075112
CACGTCT1003300.043.9186514
GCACACG1006600.043.87971511
ACGTCTG1005600.043.7846415
GTAGCAT979350.043.7388435
CGTCTGA997750.043.73227716
AGCACAC1011100.043.72496810
TAGCATC977050.043.6925336
GGTAGCA981600.043.647534
AGTCACG999200.043.64179628
GAACTCC994400.043.63526521
TCGGAAG1019900.043.617923
GTATGCC984900.043.58243645
AGAGCAC1017200.043.5721178
CACGGTA991500.043.54831731
CTCGTAT983850.043.5466242
ACTCCAG1005650.043.47821823
GATCGGA1025500.043.4555741
TCGTATG988300.043.44616343
Produced by FastQC (version 0.11.2)