FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea190.3510000004e340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea190.3510000004e340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9241567
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC6961677.532997380206193TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG740050.043.50350632
ACACGTC759600.043.42500313
CACGAGT742550.043.39851831
AGTCACG752650.043.3477428
CACACGT761600.043.32573312
CTCGTAT741450.043.2799242
CGAGTGG741450.043.27034833
CACGTCT762850.043.24294714
TCACGAG753900.043.22537230
GTATGCC746250.043.16735545
TCTCGTA743100.043.15354541
GTCACGA756200.043.11746629
GAACTCC760700.043.1137821
CAGTCAC757850.043.09186627
TCGTATG748400.043.06439243
CGTCTGA765850.043.02655416
GAGTGGA742850.043.01476734
ACGTCTG767550.042.97815715
GCACACG768800.042.9463211
CTCCAGT762000.042.92803624