FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea189.3510000004e2c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea189.3510000004e2c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9243704
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC3968544.293235698590089TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGA431100.042.89582434
CACACGT449300.042.50478412
CCGTACG436900.042.50031333
GTACGAT435800.042.42310735
ACACGTC449450.042.4205213
CACGTCT449750.042.35721214
CTCGTAT436500.042.1903642
ACCGTAC442350.042.11864532
AGTCACC445200.042.057428
TACGATC438050.042.05475236
CACCGTA444850.042.0070231
ACGTCTG453250.041.9755315
GTATGCC440050.041.93691645
TCTCGTA439600.041.8725941
GAACTCC451600.041.83000621
TCGTATG441850.041.7966343
GCACACG457400.041.78650311
CGTCTGA455350.041.77206816
CAGTCAC449650.041.7662527
CGTATGC443600.041.59623744