FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea188.3510000004e241.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea188.3510000004e241.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8649021
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG92764510.725433549068732TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG143330.1657181778145758TruSeq Adapter, Index 19 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATTTCGTATG116290.1344545238125795TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1049600.044.20285445
CACACGT1071850.044.13602412
ACACGTC1063750.044.1146913
CACGTCT1063450.044.0382814
GCACACG1077800.043.94455711
CACGTGA1060150.043.94320331
TCACGTG1059550.043.88213730
AGCACAC1083650.043.7833310
CGTCTGA1067950.043.78109416
AGTCACG1068500.043.77565828
CTCGTAT1047750.043.7633944
TCTGAAC1065500.043.72340818
GAACTCC1062450.043.71975721
ACGTCTG1070350.043.71655315
GTCACGT1064450.043.70972429
TCTCGTA1049900.043.67805543
GAGCACA1091300.043.664989
CTCCAGT1060200.043.6090824
AGAGCAC1093850.043.602278
TGAACTC1072700.043.59561520
Produced by FastQC (version 0.11.2)