FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea187.3510000004e1c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea187.3510000004e1c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7589549
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG100932613.29889299087469TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1154200.044.18911712
ACACGTC1151700.044.1463513
TCGTATG1142650.044.061345
GCACACG1157950.044.03824211
AGCACAC1159350.044.022810
GAGCACA1164300.044.011489
AGAGCAC1166700.043.994238
TCGGAAG1177600.043.9733433
GATCGGA1184250.043.851811
ATCGGAA1184750.043.7991032
TCTGAAC1158400.043.77837818
CACGTCT1160150.043.74724214
CGTCTGA1160900.043.73642316
AAGAGCA1177450.043.701497
GAACTCC1154100.043.69587721
AGTCACG1159500.043.6265428
GAAGAGC1181000.043.6048136
AACTCCA1154700.043.6010822
GTCTGAA1168650.043.58690317
TGAACTC1164400.043.58176820
Produced by FastQC (version 0.11.2)