FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea186.3510000004e142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea186.3510000004e142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10646991
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG9600.014.76490835
CCGTCCC10300.014.19833934
CACCCGT10950.014.17652931
GTCCCGA10500.013.07171436
CCCGTCC11850.012.91034133
TCACCCG13050.012.58453330
GCACACG18450.012.07150711
ACGTCTG17850.011.97314115
ACACGTC19200.011.71713613
GTCACCC12950.011.63932329
ACCCGTC13000.011.42203932
CCCGATC13250.011.03802938
CACACGT21100.010.98189812
CACGTCT20750.010.73346114
AGTCACC15850.010.36134828
CGTCTGA21200.010.08115916
GAACTCC25000.08.81878521
AGCACAC28950.08.39286310
CTCGTAT18850.08.11639344
ACTCCAG25600.08.08482423
Produced by FastQC (version 0.11.2)