FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea182.3510000004e6b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea182.3510000004e6b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19507651
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC310280.15905554184868284TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC54700.027.14658544
GTATGCC57700.025.96910745
ACGTCTG62250.025.40615315
AGTCACC58500.025.30428528
GCACACG63100.025.06391711
CTCGTAT60600.024.57785442
ACACGTC64350.024.29736713
TCGTATG63150.023.7279143
CGTCTGA67750.023.2772516
CACGTCT68050.022.97627614
CACACGT68350.022.97417312
CAGTCAC70950.021.33962227
CCAGTCA73450.021.01146726
GAACTCC75700.020.71380421
GTCACCA71500.020.67204329
ACTCCAG76000.020.39528323
TCCAGTC77100.020.104325
ACCAGAT74450.019.85395432
CCAGATC77950.019.13601733
AGCACAC84150.018.90158710
Produced by FastQC (version 0.11.2)