FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea181.3510000004e0e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea181.3510000004e0e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14214457
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC623180.4384128074677774TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC80950.037.3515129
TCACGCC83900.036.11863730
CGTATGC85400.035.645344
GCACACG88100.035.49441511
AGTCACG86250.035.3953728
GTATGCC85900.035.38543745
ACACGTC88300.035.26115813
ACGTCTG88500.035.1814715
CACGCCA86600.034.81203531
CACGTCT90750.034.3587814
CACACGT91000.034.33855412
TCGTATG89950.033.8172243
CTCGTAT89950.033.7421842
CGTCTGA92300.033.63554816
CAGTCAC95600.032.0747827
CCAGTCA97150.031.51672226
TATCTCG97400.031.21033939
ACGCCAA97200.031.13172132
CGCCAAT97600.031.00467933
GCCAATA97250.030.95540634
Produced by FastQC (version 0.11.2)