FastQCFastQC Report
Tue 22 Mar 2016
C8C2NACXX_l07n01_sea158.3510000004e8b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l07n01_sea158.3510000004e8b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24779313
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTAGC31050.016.23049731
CGTATGC37650.013.62469244
ACTAGCT37700.013.18882232
GCACACG44950.013.06282111
ACGTCTG48900.012.5597215
ACACGTC47200.012.5354513
GTATGCC41600.012.43916845
CACACGT49950.011.66515312
CACGTCT52800.011.54679714
CTCGTAT45800.011.544142
TAGCTTA44550.011.41385734
TCACTAG47100.011.32032430
TCGTATG47350.011.11868943
CGTCTGA55650.010.95545116
CTAGCTT51150.09.89685533
CCAGTCA59400.09.65787626
GCTTATC54850.09.476047536
CAGTCAC59000.09.26578427
GAACTCC65150.09.25436321
ACTCCAG65100.09.12327723
Produced by FastQC (version 0.11.2)