FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea205.3510000004e69d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea205.3510000004e69d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683025
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC19459811.562395092170348TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT213850.043.85424814
CACACGT214550.043.84877412
ACACGTC214500.043.8380213
ACGTCTG213950.043.79169515
CGTCTGA213900.043.79141616
GTATGCC214450.043.69997845
GTCTGAA215000.043.68245717
GCACACG215750.043.67787611
AGCACAC216200.043.64939510
CTCGTAT213950.043.60228742
AGTCACC213100.043.58764328
TCGTATG215200.043.5476843
TCTGAAC214900.043.52483418
CAGTCAC213700.043.48631727
CTGAACT215300.043.4335219
CCAGTCA213800.043.40284326
CGTATGC216000.043.3863944
TCTCGTA215250.043.3402441
AACTCCA214900.043.2958322
CAGATCA214550.043.27703534
Produced by FastQC (version 0.11.2)