FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea204.3510000004e581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea204.3510000004e581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230787
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC7010.3037432784342272TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1300.038.07234213
CACACGT1300.038.07234212
CTCGTAT1350.036.66225442
CACGTCT1350.036.66225414
TCGGAAG1500.034.533263
ATCTCGT1500.034.49584640
AGCACAC1450.034.13382310
TCTCGTA1450.034.13382341
AGAGCAC1450.034.1338238
GAACTCC1400.033.74593721
AGTCACG1500.032.9960328
ATCGGAA1600.032.374932
GTCACGC1600.032.33985529
TGAACTC1550.031.9316420
GCACACG1550.031.9316411
CAGTCAC1550.031.9316427
AACTCCA1550.031.9316422
ATATCTC1600.030.93377738
TATCTCG1600.030.93377739
AAGAGCA1750.030.8534287
Produced by FastQC (version 0.11.2)