FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea203.3510000004e482.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea203.3510000004e482.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4142889
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC261760.6318296242066829TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT32150.038.767742
CACACGT33400.037.98719812
ACACAGT32900.037.6163132
AGTCACA33600.037.09021428
TCGTATG33800.037.0748843
TCTCGTA33800.036.8086341
TCACACA34100.036.74471730
ACACGTC35050.036.26310713
GTCACAC35000.035.86326229
CACGTCT35500.035.6745414
CACACAG35600.035.0107231
CGTATGC35850.034.9548444
TCGGAAG36750.034.933443
CACAGTG35600.034.70269833
CAGTCAC36550.034.5274427
GTATGCC37050.033.94414545
AGTGATC36400.033.9404136
ACAGTGA36550.033.73914334
GTCTGAA37850.033.63790517
AGCACAC38150.033.55311210
Produced by FastQC (version 0.11.2)