FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea200.3510000004e5fb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea200.3510000004e5fb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642573
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC128270.7809089763438215TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15200.039.66273542
ACACGTC15750.038.85017813
CACACGT15900.038.48366512
GTCACAC15550.038.47950429
CACGTCT15950.038.3606914
CGTATGC15900.037.91657344
TCGTATG15950.037.79771843
GCACACG16350.037.56207311
GAACTCC16250.037.51406521
GTATGCC16100.037.44556445
TCTCGTA16200.037.07668741
AGTCACA16200.036.93557428
CGTCTGA16500.036.8093416
ACGTCTG16650.036.61282715
CCAGTCA16350.036.59671426
CAGTCAC16450.036.3742427
ATCTCGT16900.035.67407640
ACTCCAG17300.035.49725323
TCGGAAG17550.035.2995223
AGCACAC17650.035.178910
Produced by FastQC (version 0.11.2)