FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea180.3510000004e383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea180.3510000004e383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5522550
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC3383666.1269884383120115TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT370200.043.8491312
ACACGTC372750.043.5491613
TCGGAAG376750.043.5249563
GCACACG373450.043.49162711
GATCGGA377500.043.479061
CACGTCT373200.043.44043414
CGTCTGA371600.043.1008216
GTAGCAT368550.043.10001835
AGAGCAC378200.043.0968258
AGCACAC377300.043.07920510
GGTAGCA369050.043.07820534
AGTCACG372600.043.06440728
TAGCATC368300.043.06362536
ATCGGAA381100.043.062432
CTCGTAT369800.042.9777542
ACGTCTG376950.042.95456715
GAGCACA380000.042.8571629
TCTCGTA371400.042.80433341
TCTGAAC374300.042.7478418
CACGGTA374850.042.7385531
Produced by FastQC (version 0.11.2)