FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea179.3510000004e30c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea179.3510000004e30c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7136837
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC3441844.822640617965634TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT370200.043.36242712
ACACGTC370400.043.3450913
CACGTCT370650.043.25364314
GATCGGA375250.043.197211
TCGGAAG375200.043.1555523
GCACACG373700.043.0345611
ACGAGTG368500.042.95553632
ACGTCTG374450.042.7906715
AGTCACG370300.042.79062328
CTCGTAT368350.042.7742642
CACGAGT370300.042.75169431
CGAGTGG369350.042.73813233
CGTCTGA375000.042.6739216
AGCACAC377450.042.65558210
AGAGCAC378400.042.644218
TCGTATG370400.042.56182543
CGTATGC371100.042.487644
GTCACGA373450.042.4658329
GAGTGGA371200.042.42814334
CAGTCAC373150.042.41557727