FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea178.3510000004e284.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea178.3510000004e284.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400560
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC849441.9302997800279962TruSeq Adapter, Index 22 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC94500.042.1574713
CGTACGA93250.041.89206734
GCACACG95300.041.82718311
CACACGT95550.041.76482812
CCGTACG94000.041.62963533
CTCGTAT93550.041.10172742
ACGTCTG96600.040.98391315
CACGTCT96900.040.973114
TCGGAAG98700.040.803843
GTACGAT95500.040.78726635
GATCGGA98800.040.7839471
CACCGTA96100.040.757531
CGTCTGA97450.040.71876516
TACGATC95200.040.58672336
AGTCACC96550.040.5396328
AGCACAC99250.040.38916810
GATCTCG95500.040.30959739
CGTATGC95950.040.28468344
TCGTATG96200.040.20338443
GTATGCC96300.040.16163345
Produced by FastQC (version 0.11.2)