FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea175.3510000004e20d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea175.3510000004e20d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6459597
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2581063.9956981836482988TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG277250.043.2678735
ACACGTC283200.043.26018513
CACGTCT282650.043.2060514
GTCCCGA277650.043.20044336
CCGTCCC279250.043.1191534
GCACACG284100.043.10730711
CACACGT284600.043.0473812
ACGTCTG283600.043.0454615
CACCCGT280350.043.02692831
ACCCGTC280800.042.94859732
CCCGTCC280800.042.92921433
TCGTATG280650.042.86733245
GTCACCC281750.042.84108729
AGTCACC282300.042.83730328
CCCGATC281000.042.72347638
CGTCTGA286150.042.65400316
GATCGGA290800.042.618371
AGCACAC288500.042.5298110
CTCGTAT282900.042.51048744
TCACCCG284050.042.49551430