FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea174.3510000004e0c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea174.3510000004e0c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7198205
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC881521.2246386425504692TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTTAG96950.042.53402731
ACTTAGG98950.041.6136332
CGTATGC100300.041.2494144
CTCGTAT100350.041.1391842
GTATGCC100800.040.9778445
GCACACG102350.040.75151411
ACGTCTG102300.040.6366615
AGGCATC101100.040.6210636
ACACGTC102950.040.44845213
CACACGT103750.040.2016112
CACGTCT104300.039.87914
TCGTATG104000.039.7818843
GGCATCT103150.039.77012337
CAGTCAC104350.039.601227
CGTCTGA105150.039.51384416
CCAGTCA104800.039.4311626
TTAGGCA105450.038.9864234
ACTCCAG106600.038.9130623
TCACTTA107300.038.5334130
GAACTCC108300.038.44763621
Produced by FastQC (version 0.11.2)