FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea173.3510000004e086.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea173.3510000004e086.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834708
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG6700.011.07954729
ACACGTC9100.09.88826413
CGTATGC8150.09.10958944
AGTCACC8550.08.94539228
CCGATGT10250.08.78155833
GCACACG10100.08.68649711
ACGTCTG9450.08.56931515
CTCGTAT10150.07.97955842
CACACGT11200.07.833359212
GTATGCC9550.07.774150445
TCGTATG10500.07.713573543
CACGTCT11900.07.5611614
CGATGTA9900.07.500914634
GTATCTC11450.07.27131738
CGTCTGA11700.06.92136916
ACTCCAG13300.06.765306523
CAGTCAC12400.06.530814627
AGCACAC13200.06.4762310
CCAGTCA12250.06.427151726
TATCTCG13400.06.38011739