FastQCFastQC Report
Fri 25 Mar 2016
C8C2NACXX_l06n01_sea172.3510000004e050.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC8C2NACXX_l06n01_sea172.3510000004e050.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4820158
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC443380.9198453660647637TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51800.039.9205742
TCGTATG53150.039.033643
CACACGT54500.038.75652712
AGTCACA54050.038.4965528
ACACGTC55400.038.28932613
CACGTCT55450.038.132714
TCTCGTA54400.038.05396341
CGTATGC55100.037.6521944
GTATGCC55300.037.59739745
GCACACG56450.037.4575811
GTCACAT56350.037.16477629
CAGTCAC56200.037.0238227
CGTCTGA58050.036.3472816
ACGTCTG58250.036.29971315
AGCACAC58700.036.1754710
ACTCCAG58650.035.7453223
TCCAGTC58800.035.6927625
CCAGTCA58750.035.68484526
GAACTCC59650.035.29690621
CTCCAGT59550.035.20545224
Produced by FastQC (version 0.11.2)